Product name:DL-Dithiothreitol
CAS:27565-41-9
Molecular Formula:C4H10O2S2
Formula Weight:154.25
Specification:
Description:
Dl-dithiothreitol, also known as Dithiothreitol (DTT), is a common reducing agent, which has a strong reducing property, which is largely due to the conformational stability of its oxidation state six-membered ring (containing disulfide bond). Reduction power is affected by pH value, and can only play a reduction role when pH value is higher than 7. This is because only the negative mercaptan ions (-s -), which have been stripped of protons, are reactive, while mercaptan (-sh) is not. The pKa of sulfhydryl group is ~ 8.3.
Dl-dithiothreitol has an antioxidant effect, which can protect the reducing group on the enzyme molecule, maintain the reducing environment, and stabilize the activity of the enzyme. DTT also contains some RNA enzyme inhibitors, which can inhibit the activity of RNA enzyme. It has a similar effect to mercaptoethanol, but DTT is much less pungent and less toxic than mercaptoethanol. And when DTT was seven times less concentrated than mercaptoethanol, the effect was similar. But DTT is slightly more expensive. It is used as a proteinic acid protectant and for cleavage and sequence analysis of protein disulfide bonds.
Melting point:38-43 ºC
Boiling point:364.5 °C at 760 mmHg
Flash Point:174.2°C
Density:1.302g/cm3
Chemical property:White solid.
Product Categories:Biochemistry;O-Substituted Sugars;Sugar Alcohols;Sugars;Reagents for Electrophoresis