Product name:SP6 RNA Polymerase
Specification:500U
Description:
SP6 RNA polymerase catalyzes the synthesis of RNA. It uses a DNA template, and requires a specific SP6 phage promoter. SP6 RNA polymerase is used for in vitro transcription in a variety of applications. These include synthesis of labeled RNA probes, RNA to be used for in vitro translation, and mRNAs for biological use. SP6 DNA-dependent RNA polymerase, like T7 RNA polymerase, can be used to synthesize RNA sequences from short DNA templates which contain the 18 base pair promoter region. Use of SP6 polymerase extends the range of possible 5' sequences of RNA products since the preferred SP6 start site (of the RNA product) is 5'GAAGA, while T7 polymerase prefers 5'GGGAG. The SP6 start site can be advantageous in large-scale syntheses, where high concentrations of RNA can lead to aggregation.