Product name:2,6-dichlorophenolindophenol
CAS:956-48-9
Molecular Formula:C12H7Cl2NO2
Formula Weight:268.1
Specification:5g
Description:
2,6-dichlorophenolindophenol, ( DCPIP ) is a blue chemical compound used as a redox dye. Oxidized DCPIP is blue, reduced DCPIP is colorless.
The rate of photosynthesis can be measured by the rate at which this dye changes color (is reduced) when exposed to light in a photosynthetic system. This reaction is reversible, as colorless DCPIP can be reoxidized to blue. It is often used in measurements of the electron transport chain in plants because of its higher affinity for electrons than ferredoxin. For example, it is possible to compare rates of photosynthesis with DCPIP. Due to the reductions that occur during the light reactions, DCPIP can be substituted for NADPH, the final electron carrier in the light reactions. The light reactions will reduce the DCPIP and turn it colorless. As DCPIP is reduced and becomes colorless, the following increase in light transmittance can be measured by a spectrophotometer.