
Product name:RiboRuler High range RNA Ladder
Description:
RiboRule High Range RNA Ladder is a mixture of 8 chromatography purified single-stranded RNA transcripts (200-6000 b long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.
Ladder is available in 1 mM EDTA, pH 6.0.
RiboRuler™ High Range RNA Ladder is composed of chromatography-purified transcripts what makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use the ladder is aliquotted in smaller volumes.
RiboRuler™ High Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase, see protocol, and is ideal for Northern blots.
